Science and Research
Non-growth-based methods for the phenotypic investigation of microorganisms offer fast and label-free direct access to identity determination, vital metabolic functions and the investigation of microbial interactions with substances. Academic applications of the software-controlled, fully automated modular instrument concept GramRay.
Raman spectroscopy differs from other currently used techniques by its easy application at low cost, its high analysis speed and its broad information content both about the chemical composition and the structure of biomolecules within microorganisms.
Slight changes in the chemical composition of microorganisms can be monitored by Raman spectroscopy and used to differentiate genera, species or even strains. Pathogens can be detected from complex matrices such as soil, food and body fluids. In addition, spectroscopic studies of host-pathogen interactions and the effect of antibiotics on bacteria are also dealt with.
Academic applications use the highest sensitivity in single cell identification for the investigation of zoonoses and the detection of pathogens from beverages and food. Vibrions such as cholera are living, difficult-to-cultivate germs (VBNC) that can be reliably detected with GramRay without a previous cultivation step. Their metabolism is investigated with stable isotopes Raman (SIRM). In the elucidation of microbiomes, GramRay provides direct access to populations, as growth and incubation steps are not required. Raman also shows non PCR primer specific gaps, such as the widely used Next Generation Sequencing method for the elucidation of the microbiome.
With the GramRay system, the viability of bacteria can be examined directly. Interactions with substances or host-pathogen interactions are immediately visible. Thus the suitability of potential antibiotics for specific bacteria can be determined within only 1 hour. By visualizing the interaction of an antibiotic with the bacterial membrane, the mechanisms of antibiotic resistance are elucidated.